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plant modular cloning golden gate toolkit  (Addgene inc)


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    Structured Review

    Addgene inc plant modular cloning golden gate toolkit
    Plant Modular Cloning Golden Gate Toolkit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plant+modular+cloning+golden+gate+toolkit/MoClo+CRISPR%2FCas+Toolkit+for+Plants+(Kit+%231000000159)/pm31033104-242-16-27
    Average 93 stars, based on 2 article reviews
    plant modular cloning golden gate toolkit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).

    Mutagenesis:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).

    Construct:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).

    Clone Assay:

    Article Title: Cas9-mediated mutagenesis of potato starch-branching enzymes generates a range of tuber starch phenotypes.
    Article Snippet: .. Plasmid vectors for Cas9-mediated targeted mutagenesis were constructed according to Lawrenson et al. (2015) using the Plant Modular Cloning Golden Gate toolkit, a gift from Sylvestre Marillonnet (Addgene #1000000044; Engler et al. 2014). .. Briefly, targets were introduced into single guide RNA (sgRNA) scaffolds by PCR (for primers see Table S2) and assembled with a U6 small RNA promoter from either Arabidopsis (plCSL90002; Addgene #68261) or potato (Wang et al., 2015) into a Level 1 acceptor plasmid (plCH47751, plCH47761, plCH47772 or plCH47781).



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    93
    Addgene inc plant modular cloning golden gate toolkit
    Plant Modular Cloning Golden Gate Toolkit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plant+modular+cloning+golden+gate+toolkit/MoClo+CRISPR%2FCas+Toolkit+for+Plants+(Kit+%231000000159)/pm31033104-242-16-27
    Average 93 stars, based on 1 article reviews
    plant modular cloning golden gate toolkit - by Bioz Stars, 2026-09
    93/100 stars
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